The meaning of Sir in the Bible
(From International Standard Bible Encyclopedia)

sur: In the Old Testament this word in Genesis 43:20 the King James Version ('adhon) is changed in the Revised Version (British and American) into "my lord." In the New Testament the word sometimes represents aner, as in Acts 7:26; Acts 14:15; Acts 19:25, etc.; more frequently kurios, "lord," as in Matthew 13:27; Matthew 21:30; Matthew 27:36; John 4:11, John 4:15, John 4:19, John 4:49 (the Revised Version margin "lord"); John 20:15. In Revelation 7:14, the Revised Version (British and American) renders "my lord."

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