The meaning of Undefiled in the Bible
(From International Standard Bible Encyclopedia)

un-de-fild': In the Old Testament tam, "perfect," presents the positive side. Hence, Psalms 119:1 is translated in the Revised Version: "Blessed are they that are perfect in the way." In the New Testament amiantos, presents the negative side, "unstained" "unsullied" "without taint." Used to describe the sinlessness of Christ (Hebrews 7:26), to declare the marriage act free from all guilt, disgrace or shame (Hebrews 13:4), to contrast the heavenly inheritance with earthly possessions (I Peter 1:4).

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