The meaning of Holy of Holies in the Bible
(From Easton's Bible Dictionary)

the second or interior portion of the tabernacle. It was left in total darkness. No one was permitted to enter it except the high priest, and that only once a year. It contained the ark of the covenant only (Exodus 25:10-16). It was in the form of a perfect cube of 20 cubits. (See TABERNACLE .)

See more on the meaning of Holy Of Holies in the Bible:
Holy Of Holies {International Standard Bible Encyclopedia}

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