The meaning of Hymn in the Bible
(From Easton's Bible Dictionary)

occurs only Ephesians 5:19 and Colossians 3:16. The verb to "sing an hymn" occurs Matthew 26:30 and Mark 14:26. The same Greek word is rendered to "sing praises" Acts 16:25 (RSV, "sing hymns") and Hebrews 2:12. The "hymn" which our Lord sang with his disciples at the last Supper is generally supposed to have been the latter part of the Hallel, comprehending Psalms 113118-118. It was thus a name given to a number of psalms taken together and forming a devotional exercise.

See more on the meaning of Hymn in the Bible:
Hymn {International Standard Bible Encyclopedia}

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